Piskel!

Aseprite (previously ASE, aka Allegro Sprite Editor) is a program to create animated sprites & pixel art. Sprites are little images that can be used in your website or in a video game. You can draw characters with movement, intros, textures, patterns, backgrounds, logos, color palettes, isometric levels, and more.

Animation graphic editor easy to use - Powerful tool for spriting and pixel art.

GrafX2 is a bitmap paint program inspired by the Amiga programs Deluxe Paint and Brilliance. Specialized in 256-color drawing, it includes a very large number of tools and effects that make it particularly suitable for pixel art, game graphics, and generally any detailed graphics painted with a mouse. The program is mostly developed on Linux and Windows, but is also portable on many other platforms.

Pyxel Edit is a pixel art drawing application especially designed for working with tiles. Place tiles to form a level, edit them directly to see how they all work together, then export your tileset and the level data, and load it into your game. Tiles can even be flipped and rotated, still being editable and synced. This feature is inspired by the awesome Pixothello and Cosmigo Pro Motion, but taken one step further.

Animations: Create animations and export them as spritesheets or animated GIFs.

Pixen is a pixel art editor designed for working with low-resolution raster art, such as those 8-bit sprites found in old-school video games. With innovative features like a color palette system, patterns, and high-zoom support, Pixen packs all the tools pixel artists need in an intuitive, Mac-native interface. Pixen also makes creating frame-by-frame animations fun and easy.

Tile Studio is a complete development utility for graphics of tile-based games. The application contains a bitmap editor for creating tiles and sprites and a map editor for designing level maps.Tile Studio can be used together with ANY programming language, since the output is completely programmable! You can program Tile Studio to output your maps, animation sequences, bitmaps, palettes, etc. in any format and include the output directly into your source code!

Moai is a spiritual successor to one of the elder gods of pixel editing: Autodesk Animator. Designed to make detailed pixel work easy while preserving many of Animator's unique processes, Moai exists to bridge the old with the new. The only things not returning are the FLI/FLC and 8bpp support.

Pixelsque's goal is to be as simple as possible, while still retaining many powerful features. It works like any standard pixel art editor, with a pen, eraser, and shapes. Pinch zoom and two finger drag can be used to move around the image from anywhere. Lesser-used functions such as save/open/new, and others can be found in the menu, saving valuble screen space on phones, and allowing easy access on tablets. It fully supports all Android devices from low-end phones to high-end tablets.

Pro Motion is a drawing and animation software for Windows designed similar to the famous Amiga Deluxe Paint (DPaint).

mtPaint is a painting program which Mark Tyler developed from scratch so he could easily create pixel art and manipulate digital photos.

Pixel art image editor, created for full keyboard control of image drawing without mouse using. It can be convenient to use it when you place notebook on your knees during travel in public transport (subway, buses, trains).

IconLover is a powerful, yet easy to use icon editor for making your own icons for any operating system, including Windows, Mac OS, UNIX/Linux and iPhone.

Poxi is a browser-based, pixel art editor

PikoPixel is a free application for drawing & editing pixel-art images.