Jpixel!

Aseprite (previously ASE, aka Allegro Sprite Editor) is a program to create animated sprites & pixel art. Sprites are little images that can be used in your website or in a video game. You can draw characters with movement, intros, textures, patterns, backgrounds, logos, color palettes, isometric levels, and more.

Remember the good old 2D games and their beautiful sprites? With Piskel you can create your own in a few clicks.

Animation graphic editor easy to use - Powerful tool for spriting and pixel art.

Pyxel Edit is a pixel art drawing application especially designed for working with tiles. Place tiles to form a level, edit them directly to see how they all work together, then export your tileset and the level data, and load it into your game. Tiles can even be flipped and rotated, still being editable and synced. This feature is inspired by the awesome Pixothello and Cosmigo Pro Motion, but taken one step further.

Spriter : an intuitive 2D animation tool for video game makers.

Pixen is a pixel art editor designed for working with low-resolution raster art, such as those 8-bit sprites found in old-school video games. With innovative features like a color palette system, patterns, and high-zoom support, Pixen packs all the tools pixel artists need in an intuitive, Mac-native interface. Pixen also makes creating frame-by-frame animations fun and easy.

Pixaki is a powerful pixel art creation app for iPad. It features many of the same features found in desktop apps — such as custom canvas sizes, layers, precise colour selection and customisable palettes — but with a gorgeous multi-touch interface that is just right for iPad. Whether you're creating 8-bit game characters or vast isometric cityscapes, Pixaki has the tools you'll need in an app you'll love.

Pixelsque's goal is to be as simple as possible, while still retaining many powerful features. It works like any standard pixel art editor, with a pen, eraser, and shapes. Pinch zoom and two finger drag can be used to move around the image from anywhere. Lesser-used functions such as save/open/new, and others can be found in the menu, saving valuble screen space on phones, and allowing easy access on tablets. It fully supports all Android devices from low-end phones to high-end tablets.

Sprite Something is a drawing, animation and tilemap application for pixel artists and game developers. Draw multiple frames individually, then quickly drag and drop frames into an animation, or use your frames to draw tilemaps for your game levels. Export your creations to PNG, animated GIF's and CSV files (tilemaps).

Dotpict is a simple, intuitive and effective pixel art editor designed for mobile use.

Poxi is a browser-based, pixel art editor

Kenney Studio is an easy to use tool to create game sprites using various pre-made objects. Each object has unique options and without any artistic skill you can create a wide range of sprites.