Sprite Something!

Animations: Create animations and export them as spritesheets or animated GIFs.

Pixen is a pixel art editor designed for working with low-resolution raster art, such as those 8-bit sprites found in old-school video games. With innovative features like a color palette system, patterns, and high-zoom support, Pixen packs all the tools pixel artists need in an intuitive, Mac-native interface. Pixen also makes creating frame-by-frame animations fun and easy.

Pixelsque's goal is to be as simple as possible, while still retaining many powerful features. It works like any standard pixel art editor, with a pen, eraser, and shapes. Pinch zoom and two finger drag can be used to move around the image from anywhere. Lesser-used functions such as save/open/new, and others can be found in the menu, saving valuble screen space on phones, and allowing easy access on tablets. It fully supports all Android devices from low-end phones to high-end tablets.

Spriter : an intuitive 2D animation tool for video game makers.

Pixaki is a powerful pixel art creation app for iPad. It features many of the same features found in desktop apps — such as custom canvas sizes, layers, precise colour selection and customisable palettes — but with a gorgeous multi-touch interface that is just right for iPad. Whether you're creating 8-bit game characters or vast isometric cityscapes, Pixaki has the tools you'll need in an app you'll love.

Kenney Studio is an easy to use tool to create game sprites using various pre-made objects. Each object has unique options and without any artistic skill you can create a wide range of sprites.